Disinfection is usually carried out by using disinfectants (chemicals). Some disinfectants may be very effective and have a wide spectrum (have the capability of destroying a wide variety of microorganism) while others may have a narrow spectrum but, they may be easy to use, be non toxic or inexpensive. 

Sterilization can be done by three methods: physical, chemical and physiochemical. Physical method includes heat, radiation, and filtration. Chemical methods involve using liquid and gaseous chemicals. Physiochemical is a combination of physical and chemical method.

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